A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres.
نویسندگان
چکیده
We describe a simple, multiplexed assay that integrates glycan synthesis, bioconjugation to microspheres, fluorescent chemical/biochemical detection and multiparameter flow cytometric analysis to screen activities of different families of carbohydrate-active enzymes.
منابع مشابه
Correlation Index-Based Responsible-Enzyme Gene Screening (CIRES), a Novel DNA Microarray-Based Method for Enzyme Gene Involved in Glycan Biosynthesis
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عنوان ژورنال:
- Chemical communications
دوره 49 48 شماره
صفحات -
تاریخ انتشار 2013